SOP FOR Uv LIGHT EFFICACY TEST

SOP FOR Uv LIGHT EFFICACY TEST

OBJECTIVE: Objective of this SOP is to provide guidelines for monitoring UV light efficiency in LAF and pass box.

SCOPE: This procedure is applicable for UV light efficiency test in Microbiology laboratory

REFERENCE: In house

RESPONSIBILITY:
Microbiologist Oficer: follow as per SOP.
Q.C Head: to ensure the proper execution of the SOP.
Q.A: to ensure that the SOP is Correclty follow or not.

DISTRIBUTION:

Sr. no Particular Departments
QA QC PD ST MB EHS PR MT
1 Master copy no. 01
2 Control copy no. 01
3 Total control copies 01

ABBREVIATION:
N.A: Not applicable
Q.C: Quality control
Q.A: Quality assurance
UV: Ultra violet
LAF: Laminar air flow
SCDA: Soyabean casein digest agar medium
Cfu: colony forming unit

PROCEDURE:

1. Prepare SCDA medium as per SOP no. SOP/P/MB/15.
2. Aseptically pour approx. 15-20 ml of sterile molten cooled (40-45°C) SCDA agar into sterile 90 mm petri plates under LAF.
3. Allow media to solidify the plates under LAF, after solidification label all the plates with the name of media, preparation batch no. and date of preparation.
4. Invert and incubate the plates at 30-35°C for 24 hrs in BOD incubator. After incubation, check plates for any contamination.
5. If there is contamination, discard the plates as per SOP No. SOP/P/MB/09.
6. After pre-incubation, transfer 0.1 ml of not less than 105 cfu/ml culture of B. subtilis to two SCDA petri plates and spread the colony using sterile spreader (prepare 105 B. subtilis cfu/ml suspension).
Now wrap one petri plate containing subtilis in aluminum foil and don’t wrapping with other petri plate. petri plates transfer in LAF.
7. Now switch on UV light for 30 min.
8. After 30 min exposure, switch off the UV light and close the lid of unwrapped petri plate.
9. Incubate both the petri plates in incubator at 30-35°C for 24-48 hrs.
10. Prepare positive control by streaking B. subtilis culture and negative control uninoculated.
11. After incubation observe the plate for total bacterial count. Record the results in the annexure given below.
12. Acceptance criteria: No growth (nil cfu/ml) should be observed in the plate exposed to UV light for 30 min. when compared to the plate wrapped with aluminum foil (unexposed plate).

FREQUENCY: Quarterly

ANNEXURE:
1. UV Light efficacy test

Annexure: I- UV Light efficacy test

Name of instrument: Autoclave load no.:
Date of analysis: Date of reporting:
UV light switched on at: UV light switched off at:
Standard culture: Bacillus subtilis (105 cfu/ml) In house batch no.:
Medium used: Soyabean casein digest agar incubated at 30-35°C for 24-48 hrs
Batch no.:

Observation:

Sr. no Plate wrapped with aluminum foil
Plate unwrapped
(Limit: NIL) Positive control
(Limit:105cfu/ml) Negative control
(Limit: NIL)